Regulation of JC virus by the POU-domain transcription factor Tst-1: implications for progressive multifocal leukoencephalopathy.

Abstract

Progressive multifocal leukoencephalopathy results from an opportunistic infection of myelin-producing oligodendrocytes by the glia-specific human papovavirus JC. In this report, evidence is presented that the glial transcription factor Tst-1, a member of the POU-domain family, stimulates transcription of both early and late viral genes. Stimulation was dependent on site-specific binding of Tst-1 to the JC viral regulatory region and on the presence of an intact amino-terminal transactivation domain within Tst-1. Because of its ability to increase the expression of viral large tumor antigen, Tst-1 stimulated viral DNA replication, without participating directly in the replication event. Our results suggest that Tst-1 is one of the determining factors in the glia specificity of JC virus.

• Authors: M Wegner, D W Drolet, M G Rosenfeld
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 90 Issue 10 Pg. 4743-7 (May 15 1993) ISSN: 0027-8424 [Print] United States
• PMID: 8389455 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• DNA-Binding Proteins
• Nerve Tissue Proteins
• Oligodeoxyribonucleotides
• POU3F1 protein, human
• Transcription Factors
• Viral Proteins
• Viral Structural Proteins
• Octamer Transcription Factor-6

Topics

• Base Sequence
• DNA-Binding Proteins (genetics)
• Gene Expression Regulation, Viral
• Genes, Viral
• Humans
• In Vitro Techniques
• JC Virus (genetics, growth & development)
• Leukoencephalopathy, Progressive Multifocal (genetics)
• Molecular Sequence Data
• Nerve Tissue Proteins (genetics)
• Octamer Transcription Factor-6
• Oligodeoxyribonucleotides (chemistry)
• Transcription Factors (genetics)
• Transcription, Genetic
• Tumor Cells, Cultured
• Viral Proteins (genetics)
• Viral Structural Proteins (genetics)