Feline CD4 molecules expressed on feline non-lymphoid cell lines are not enough for productive infection of highly lymphotropic feline immunodeficiency virus isolates.

Abstract

To investigate whether the feline CD 4 (fCD4) molecules are involved in infections of highly lymphotropic feline immunodeficiency virus (FIV) isolates, we expressed fCD4 stably on Crandell feline kidney cells and Felis catus whole foetus 4 cells by transfection of a cDNA encoding the fCD4 glycoprotein, and then infected them with TM 1 and TM 2 strains of FIV, which are unable to infect these cells productively. In spite of fCD 4 being expressed on these cells, no virus production was observed. This result indicates that fCD 4 expression alone cannot induce a productive infection of the FIV TM 1 and TM 2 strains.

• Authors: J Norimine, T Miyazawa, Y Kawaguchi, K Tomonaga, Y S Shin, T Toyosaki, M Kohmoto, M Niikura, Y Tohya, T Mikami
• Journal: Archives of virology (Arch Virol) Vol. 130 Issue 1-2 Pg. 171-8 ( 1993) ISSN: 0304-8608 [Print] Austria
• PMID: 8389115 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CD4 Antigens
• DNA, Viral
• Oligodeoxyribonucleotides
• Recombinant Proteins

Topics

• Animals
• Base Sequence
• CD4 Antigens (biosynthesis, genetics, immunology)
• Cats (immunology)
• Cell Line
• DNA, Viral (genetics)
• Fetus
• Flow Cytometry
• Fluorescent Antibody Technique
• Genes, gag
• Immunodeficiency Virus, Feline (genetics, immunology, physiology)
• Kidney
• Molecular Sequence Data
• Oligodeoxyribonucleotides
• Plasmids
• Polymerase Chain Reaction
• Recombinant Proteins (biosynthesis, immunology)
• Transfection