Abstract |
The effect of pituitary adenylate cyclase activating polypeptide ( PACAP) on small cell lung cancer (SCLC) cells was investigated. In Fura 2-AM loaded NCI-N417 cells, PACAP-27 or PACAP-38 elevated cytosolic calcium in a dose-dependent manner. The cytosolic calcium was elevated from 133 to 185 nM with PACAP-27 (100 nM). Because PACAP-27 strongly elevated cytosolic calcium after the addition of 1 mM EGTA, PACAP released calcium from intracellular pools using NCI-N417 cells; similar results were obtained for SCLC cell line NCI-H345. Pituitary adenylate cyclase activating polypeptide-27 inhibited 125I-VIP and 125I-PACAP binding to NCI-N417 cells with high affinity (IC50 = 30 and 5 nM), whereas VIP inhibited 125I-VIP and 125I-PACAP-27 binding with IC50 values of 5 and 200 nM. Both 100 nM VIP and PACAP-27 elevated cAMP levels in NCI-H345 cells. In contrast, 1 microM VIP had no effect on cytosolic calcium, whereas 100 nM PACAP-27 caused a strong calcium response. Both 100 nM VIP and 10 nM PACAP-27 significantly stimulated the clonal growth of NCI-H345 cells. These data suggest that biologically active VIP and PACAP receptors are present on SCLC cells.
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Authors | T W Moody, F Zia, A Makheja |
Journal | Peptides
(Peptides)
1993 Mar-Apr
Vol. 14
Issue 2
Pg. 241-6
ISSN: 0196-9781 [Print] United States |
PMID | 8387187
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- ADCYAP1 protein, human
- Neuropeptides
- Pituitary Adenylate Cyclase-Activating Polypeptide
- Receptors, Cell Surface
- Receptors, Gastrointestinal Hormone
- Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide
- Receptors, Pituitary Hormone
- Receptors, Vasoactive Intestinal Peptide
- Vasoactive Intestinal Peptide
- Cyclic AMP
- Calcium
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Topics |
- Amino Acid Sequence
- Calcium
(metabolism)
- Carcinoma, Small Cell
(metabolism)
- Cyclic AMP
(metabolism)
- Humans
- Lung Neoplasms
(metabolism)
- Molecular Sequence Data
- Neuropeptides
(chemistry, metabolism, pharmacology)
- Pituitary Adenylate Cyclase-Activating Polypeptide
- Receptors, Cell Surface
(drug effects, metabolism)
- Receptors, Gastrointestinal Hormone
(metabolism)
- Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide
- Receptors, Pituitary Hormone
- Receptors, Vasoactive Intestinal Peptide
- Tumor Cells, Cultured
(drug effects, metabolism)
- Vasoactive Intestinal Peptide
(chemistry, metabolism)
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