Effects of hyaluronic acid on macrophage phagocytosis and active oxygen release.

Abstract

The effects of hyaluronic acid (HA) on macrophage function, in terms of the phagocytosis of latex beads and superoxide anion and hydrogen peroxide release stimulated by phorbol myristate acetate (PMA), were studied in guinea-pig peritoneal macrophages. Phagocytosis was inhibited in a dose- and molecular-weight-dependent manner by HA. The addition of PMA to the culture, at a dose of more than 10 ng ml-1, caused an increase in the release of active oxygens. The release of active oxygens was inhibited by high molecular weight HA (MW 2.02 x 10(6), HA-202) in a dose-dependent manner. In cell-free systems, HA-202 had a negligible effect in scavenging these active oxygens. Of the three molecular sizes of HA (MW: 0.28 x 10(6), 0.98 x 10(6) and 2.02 x 10(6)), HA-202 most strongly inhibited the active oxygen release. These results indicate that high-molecular-weight HA acts directly on macrophages to inhibit phagocytosis and active oxygen formation, which, in turn, ameliorates the progression of chronic inflammation.

• Authors: Y Suzuki, T Yamaguchi
• Journal: Agents and actions (Agents Actions) Vol. 38 Issue 1-2 Pg. 32-7 (Jan 1993) ISSN: 0065-4299 [Print] Switzerland
• PMID: 8386900 (Publication Type: Journal Article)

Chemical References

• Reactive Oxygen Species
• Xanthines
• Superoxides
• Xanthine
• Hyaluronic Acid
• Hydrogen Peroxide
• Xanthine Oxidase
• Tetradecanoylphorbol Acetate

Topics

• Animals
• Cells, Cultured
• Dose-Response Relationship, Drug
• Guinea Pigs
• Hyaluronic Acid (pharmacology)
• Hydrogen Peroxide (metabolism)
• Macrophages (drug effects, immunology)
• Male
• Molecular Weight
• Peritoneal Cavity
• Phagocytosis (drug effects)
• Plastic Embedding
• Reactive Oxygen Species (metabolism)
• Superoxides (metabolism)
• Tetradecanoylphorbol Acetate (pharmacology)
• Xanthine
• Xanthine Oxidase (metabolism)
• Xanthines (metabolism)