An anti-okadaic acid-anti-idiotypic antibody bearing an internal image of okadaic acid inhibits protein phosphatase PP1 and PP2A catalytic activity.

Abstract

Okadaic acid (OA), produced by marine phytoplankton, is the parent compound of a family of marine toxins responsible for diarrheic shellfish poisoning (DSP). A monoclonal antibody to OA (6/50) (Ab1) has been raised and in turn used for immunization of syngeneic animals. Mice inoculated with the 6/50 idiotype produced both anti-idiotypic antibodies (Ab2) and OA binding antibodies (Ab3). The selected anti-idiotypic antibody 1/59 bound to the immunizing 6/50 idiotype but not to F(ab')2 fragments of pooled normal mouse Ig. It inhibited the binding of OA to solid-phase attached F(ab')2 of 6/50 IgG as well as the binding of 6/50 IgG to a solid-phase bound OA. Like OA, 1/59 anti-idiotypic antibody inhibited protein phosphatase 1 and 2A catalytic subunits in a 32P-phosphorylase a phosphatase radioassay. Thus, 1/59 IgG is a novel internal image anti-idiotypic antibody (Ab2 beta) and can serve as a surrogate of OA in biological assays.

• Authors: W S Shestowky, C F Holmes, T Hu, J Marr, J L Wright, J Chin, H M Sikorska
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 192 Issue 1 Pg. 302-10 (Apr 15 1993) ISSN: 0006-291X [Print] United States
• PMID: 8386509 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Anti-Idiotypic
• Ethers, Cyclic
• Isoenzymes
• Okadaic Acid
• Phosphoprotein Phosphatases
• Protein Phosphatase 1

Topics

• Animals
• Antibodies, Anti-Idiotypic (immunology, metabolism)
• Catalysis
• Ethers, Cyclic (immunology)
• Hybridomas
• Isoenzymes (antagonists & inhibitors)
• Mice
• Okadaic Acid
• Phosphoprotein Phosphatases (antagonists & inhibitors)
• Protein Phosphatase 1
• Tumor Cells, Cultured