[Macromolecular biochemistry of normal and pathological white blood cells in man].

Abstract

Leucocytes from normal donors and leukemia patients were isolated and lebelled in vitro with 32P-orthophosphate in order to compare labelling characteristics of nuclear high-molecular weight RNA, labelling characteristics, nucleotide compositions and oligonucleotide frequencies of ribosomal 28 S RNA. These studies revealed 1. structural microheterogeneity of 28 S RNA between the various leukemia cells studies without presenting a leukemia-specific structural marker, 2. an impaired production of ribosomal 28 S RNA from its nuclear precursor 45 S RNA in acute myeloblastic leukemia compared to PHA-stimulated normal lymphocytes. In the second part of this work, the influence of RNA from immunocompetent lymphocytes on the PHA-stimulation of M. Hodgkin lymphocytes was analyzed; the third part deals with studies on macromolecular carriers forcytostatic anthracyclines in human leukemia cells.

• Authors: S Seeber
• Journal: Fortschritte der Medizin (Fortschr Med) Vol. 95 Issue 5 Pg. 295-8 (Feb 03 1977) ISSN: 0015-8178 [Print] Germany
• Vernacular Title: Untersuchungen zur makromolekularen Biochemie normaler und pathologischer weisser Blutzellen des Menschen
• PMID: 838449 (Publication Type: English Abstract, Journal Article)

Chemical References

• Macromolecular Substances
• Phosphorus Radioisotopes
• RNA, Neoplasm
• RNA, Ribosomal

Topics

• Hodgkin Disease (analysis, pathology)
• Humans
• In Vitro Techniques
• Isotope Labeling
• Leukemia (analysis, pathology)
• Leukocytes (analysis)
• Macromolecular Substances (analysis)
• Phosphorus Radioisotopes
• RNA, Neoplasm (analysis)
• RNA, Ribosomal (analysis)