Molecular characterization of receptors for human interleukin-8, GRO/melanoma growth-stimulatory activity and neutrophil activating peptide-2.

Abstract

Interleukin-8 (IL-8), neutrophil activating peptide-2 (NAP-2), and growth regulated gene (GRO, also known as melanoma growth stimulatory activity) are members of a family of peptides which are chemotactic agents for inflammatory cells such as neutrophils. Receptors have been identified for IL-8, GRO and NAP-2 on human neutrophils and granulocytic cell lines, and it has been observed that these cytokines can cross-compete for binding to a common receptor. Using the recently characterized rabbit IL-8 receptor as a probe, two classes of cDNAs, termed type 1 and type 2, were isolated from a human neutrophil library. The type 1 receptor binds only IL-8 while the type 2 receptor binds IL-8, GRO and NAP-2 at high affinity when respective cDNAs are expressed in COS-7 cells. The two cDNAs encode proteins that have an amino acid sequence identity of 77% while the type 1 and 2 receptors have an identity of 84 and 74% with the rabbit IL-8 receptor. These receptors also show significant homology with receptors for other chemotactic agents and with potential coding regions from the human cytomegalovirus genome.

• Authors: D P Cerretti, C J Kozlosky, T Vanden Bos, N Nelson, D P Gearing, M P Beckmann
• Journal: Molecular immunology (Mol Immunol) Vol. 30 Issue 4 Pg. 359-67 (Mar 1993) ISSN: 0161-5890 [Print] England
• PMID: 8384312 (Publication Type: Journal Article)

Chemical References

• CXCL1 protein, human
• Chemokine CXCL1
• Chemokines, CXC
• Chemotactic Factors
• Cxcl1 protein, mouse
• Growth Substances
• Intercellular Signaling Peptides and Proteins
• Interleukin-8
• Neoplasm Proteins
• PPBP protein, human
• Peptides
• Receptors, Cell Surface
• Receptors, Cytokine
• Receptors, Immunologic
• Receptors, Interleukin-8A
• beta-Thromboglobulin
• melanoma growth stimulating activity receptor
• connective tissue-activating peptide
• DNA

Topics

• Amino Acid Sequence
• Animals
• Cell Line
• Cells, Cultured
• Chemokine CXCL1
• Chemokines, CXC
• Chemotactic Factors (metabolism)
• DNA (isolation & purification)
• Dose-Response Relationship, Drug
• Granulocytes (metabolism)
• Growth Substances (metabolism)
• Humans
• Intercellular Signaling Peptides and Proteins
• Interleukin-8 (metabolism)
• Leukemia, Myeloid
• Mice
• Molecular Sequence Data
• Neoplasm Proteins (metabolism)
• Neutrophils (metabolism)
• Peptides (metabolism)
• Receptors, Cell Surface (chemistry, metabolism)
• Receptors, Cytokine
• Receptors, Immunologic (chemistry, metabolism)
• Receptors, Interleukin-8A
• Sequence Alignment
• Sequence Homology, Amino Acid
• Tumor Cells, Cultured
• beta-Thromboglobulin