Protein C activator from the venom of Agkistrodon blomhoffi ussuriensis retards thrombus formation in the arterio-venous shunt in rats.

Abstract	Protein C (PC) is an anticoagulant protein which, being activated by thrombin, degrades factors V/Va and VIII/VIIIa and releases a tissue-type plasminogen activator. Some Agkistrodon snake venoms contain PC activators which, in experiments, exert an anticoagulant action. An antithrombotic effect of the PC activator from the venom of A. blomhoffi ussuriensis on the model of thrombus formation in the arterio-venous shunt in rats was under investigation. Administration of the PC activator resulted in a dose-dependent prolongation of the thrombus formation time and a decrease in plasma PC activity, which were accompanied by a decrease in factor V activity and APTT prolongation. No reliable changes in the t-PA level, ADP- and epinephrine-induced platelet aggregation were observed. Platelet adhesion to glass beads diminished. We assume that the antithrombotic effect of the PC activator from the A. blomhoffi venom in the platelet-dependent thrombosis model is caused by PC activation and subsequent factor V inactivation as well as by platelet adhesiveness reduction.
Authors	A E Kogan, G V Bashkov, I D Bobruskin, E P Romanova, V A Makarov, S M Strukova
Journal	Thrombosis research (Thromb Res) Vol. 70 Issue 5 Pg. 385-93 (Jun 01 1993) ISSN: 0049-3848 [Print] United States
PMID	8378894 (Publication Type: Journal Article)
Chemical References	Crotalid Venoms Fibrinolytic Agents Intercellular Signaling Peptides and Proteins Peptides Protein C snake venom protein C activator Factor V
Topics	Amino Acid Sequence Animals Arteriovenous Shunt, Surgical Blood Coagulation (drug effects) Chromatography, High Pressure Liquid Crotalid Venoms (chemistry) Dose-Response Relationship, Drug Enzyme Activation (drug effects) Factor V (metabolism) Fibrinolytic Agents (pharmacology, therapeutic use) Intercellular Signaling Peptides and Proteins Male Molecular Sequence Data Partial Thromboplastin Time Peptides (isolation & purification, pharmacology, therapeutic use) Platelet Adhesiveness (drug effects) Platelet Aggregation (drug effects) Protein C (metabolism) Rats Rats, Wistar Species Specificity Thrombosis (prevention & control)

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