Abstract |
The serum 4-O-methylpyridoxine (MPN) levels in a 21 months-old patient with gin-nan food poisoning were determined by HPLC. The blood of the patient was taken at 8.5 and 15.5 h after taking about 50 ginkgo albumens. After deproteinization of the serum, the supernatant was pretreated with Sep-pak C18 cartridge and was applied to HPLC. HPLC was performed with a Hibar LiChrosorb RP-18 (4.0 mm i.d. x 250 mm, 7 microns) using a fluorescence detector (wave length of excitation and fluorescence; 290 and 400 nm, respectively). The serum MPN level was determined by the absolute calibration method. The determination limit of MPN in the serum was 0.05 micrograms/ml. The serum MPN concentration was 0.09 micrograms/ml at 8.5 h after taking ginkgo seeds, and was less than the determination limit of MPN (0.05 micrograms/ml) at 15.5 h. MPN, isolated from the seed of Ginkgo biloba L., is responsible for "gin-nan food poisoning," and its cardinal symptoms are mainly tonic and/or clonic convulsions and loss of consciousness. Infants are particularly vulnerable. This method may be available for proving the gin-nan food poisoning, chemically.
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Authors | M Yagi, K Wada, M Sakata, M Kokubo, M Haga |
Journal | Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan
(Yakugaku Zasshi)
Vol. 113
Issue 8
Pg. 596-9
(Aug 1993)
ISSN: 0031-6903 [Print] Japan |
PMID | 8377108
(Publication Type: Case Reports, English Abstract, Journal Article)
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Chemical References |
- 4-methoxymethylpyridoxine
- Pyridoxine
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Topics |
- Chromatography, High Pressure Liquid
- Humans
- Infant
- Plant Poisoning
(blood)
- Plants, Edible
- Pyridoxine
(analogs & derivatives, blood)
- Seizures
(etiology)
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