Morphological changes of Candida albicans induced by BMY-28864, a highly water-soluble pradimicin derivative.

Abstract

The time course of BMY-28864-dependent morphological changes in Candida albicans A9540 was studied by electron microscopy. Scanning electron microscopy revealed that BMY-28864 often induced cell surface deformations such as abnormal swelling and bulging around budding sites and bud scars. The cell membrane damage was visualized by freeze-fracturing technique as deep pit-like invaginations. Transmission electron microscopy with thin-sectioned specimens also demonstrated that BMY-28864 induced cell membrane invaginations together with cell membrane detachment from the cell wall, nuclear membrane fragmentation and mitochondrial aberration. Statistical sequence analysis of the prominent BMY-28864-dependent morphological changes of the yeast cells led to the conclusion that BMY-28864 first attacked the cell membrane and then caused disintegration of other intracytoplasmic organelles, resulting in the lethal effect.

• Authors: K Numata, T Ueki, N Naito, N Yamada, N Kamasawa, T Oki, M Osumi
• Journal: Journal of electron microscopy (J Electron Microsc (Tokyo)) Vol. 42 Issue 3 Pg. 147-55 (Jun 1993) ISSN: 0022-0744 [Print] Japan
• PMID: 8376921 (Publication Type: Journal Article)

Chemical References

• Anthracyclines
• Antibiotics, Antineoplastic
• Antifungal Agents
• Water
• pradimicin A
• N,N-dimethylpradimicin FA-2

Topics

• Anthracyclines
• Antibiotics, Antineoplastic (pharmacology)
• Antifungal Agents (pharmacology)
• Candida albicans (drug effects, ultrastructure)
• Cell Membrane (drug effects, ultrastructure)
• Freeze Fracturing
• Microscopy, Electron (methods)
• Microscopy, Electron, Scanning
• Mitochondrial Swelling (drug effects)
• Nuclear Envelope (drug effects, ultrastructure)
• Solubility
• Water