Abstract |
Lissencephaly ( agyria- pachygyria) is a human brain malformation manifested by a smooth cerebral surface and abnormal neuronal migration. Identification of the gene(s) involved in this disorder would facilitate molecular dissection of normal events in brain development. Type 1 lissencephaly occurs either as an isolated abnormality or in association with dysmorphic facial appearance in patients with Miller-Dieker syndrome. About 15% of patients with isolated lissencephaly and more than 90% of patients with Miller-Dieker syndrome have microdeletions in a critical 350-kilobase region in chromosome 17p13.3 (ref. 6). These deletions are hemizygous, so haplo-insufficiency for a gene in this interval is implicated. Here we report the cloning of a gene (LIS-1, lissencephaly-1) in 17p13.3 that is deleted in Miller-Dieker patients. Non-overlapping deletions involving either the 5' or 3' end of the gene were found in two patients, identifying LIS-1 as the disease gene. The deduced amino-acid sequence shows significant homology to beta-subunits of heterotrimeric G proteins, suggesting that it could possibly be involved in a signal transduction pathway crucial for cerebral development.
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Authors | O Reiner, R Carrozzo, Y Shen, M Wehnert, F Faustinella, W B Dobyns, C T Caskey, D H Ledbetter |
Journal | Nature
(Nature)
Vol. 364
Issue 6439
Pg. 717-21
(Aug 19 1993)
ISSN: 0028-0836 [Print] England |
PMID | 8355785
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
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Topics |
- Amino Acid Sequence
- Animals
- Brain
(abnormalities)
- Chromosomes, Human, Pair 17
- Cloning, Molecular
- Congenital Abnormalities
(genetics)
- GTP-Binding Proteins
(genetics)
- Humans
- Hybrid Cells
- In Situ Hybridization, Fluorescence
- Mice
- Molecular Sequence Data
- Repetitive Sequences, Nucleic Acid
- Sequence Homology, Amino Acid
- Signal Transduction
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