Reduction of experimental myocardial infarct size by infusion of lactosylphenyl Trolox.

Abstract

OBJECTIVE:

The aim was to determine if lactosylphenyl Trolox could improve myocardial resistance to ischaemia and reperfusion. Lactosylphenyl Trolox is derived by coupling p-aminophenyl-beta-D-lactopyranoside to Trolox. Trolox, a polar analogue of vitamin E, has been found to protect human cardiomyocytes against oxyradicals and to reduce myocardial damage by 66% in a canine ischaemia-reperfusion model.

METHODS:

New Zealand white rabbits (weighing approximately 3.5 kg) were subjected to 1 h ischaemia by ligation of the main branch of the anterior ventricular coronary artery. Approximately 1-2 min before release of ischaemia, a 30 ml bolus of saline (placebo control) or saline containing lactosylphenyl Trolox was injected into the right external jugular vein, followed by 3 h reperfusion. The area at risk was identified by staining with Evans Blue. Area of necrosis was indicated by tetrazolium red staining, confirmed by histopathology and quantified by planimetry.

RESULTS:

The control group (n = 6) had 46.6(SD 10.0)% necrosis of the area at risk but the lactosylphenyl Trolox treated groups (n = 6 per group) had reduced necrosis: 34.0(6.5)%, 17.4(8.2)%, and 6.9(3.6)% at doses of 2.5, 5.0, and 10 mumol.kg-1, respectively (each with p < 0.05 v control value). These translated to 48.6(14.0)%, 62.7(17.6)%, and 85.3(7.8)% myocardial salvage, respectively. In contrast, the salvages achieved with 2.5 and 10 mumol.kg-1 of Trolox were 31.0(11.0)% and 62.1(18.9)% respectively (both p < 0.05 v lactosylphenyl Trolox).

CONCLUSIONS:

Lactosylphenyl Trolox reduces myocardial infarct size more effectively than Trolox in a rabbit model of ischaemia-reperfusion.

• Authors: T W Wu, J Wu, L H Zeng, H Sugiyama, D A Mickle, J X Au
• Journal: Cardiovascular research (Cardiovasc Res) Vol. 27 Issue 5 Pg. 736-9 (May 1993) ISSN: 0008-6363 [Print] England
• PMID: 8348572 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Chromans
• Glycosides
• Tetrazolium Salts
• lactosylphenyl-trolox

Topics

• Animals
• Chemical Phenomena
• Chemistry
• Chromans (therapeutic use)
• Disease Models, Animal
• Glycosides (therapeutic use)
• Histocytochemistry
• Male
• Myocardial Infarction (drug therapy, pathology)
• Myocardial Reperfusion Injury (pathology, prevention & control)
• Myocardium (pathology)
• Rabbits
• Tetrazolium Salts