[Lectin histochemistry of choroidal malignant melanoma].

Abstract

Tumor specimens of malignant choroidal melanoma from a 50 year-old man were studied with reference to labeling with fluorescence-labeled lectins: wheat germ agglutinin (WGA), specific for sialic acid and N-acetylglucosamine, and Ricinus communis agglutinin-1 (RCA-1), specific for galactose. Epithelioid cells occupying the apex and internal portion of a dome-shaped tumor had intense labeling of WGA but little binding to RCA-1. Spindle-shaped cells in the base of the tumor were labeled moderately with WGA and faintly with RCA-1. Neuraminidase treatment of the specimens led to a marked increase in the RCA-1 binding to both epithelioid and spindle-shaped tumor cells. The results suggest association of the malignancy of choroidal melanoma cells with oligosaccharides of cell membrane-bound glycoconjugates.

• Authors: M Sugata, F Uehara, Y Nakashima, N Ohba
• Journal: Nippon Ganka Gakkai zasshi (Nippon Ganka Gakkai Zasshi) Vol. 97 Issue 6 Pg. 747-51 (Jun 1993) ISSN: 0029-0203 [Print] Japan
• PMID: 8328344 (Publication Type: Case Reports, English Abstract, Journal Article)

Chemical References

• Antigens, Tumor-Associated, Carbohydrate
• Lectins
• Oligosaccharides
• Plant Lectins
• Ricinus communis agglutinin-1
• Wheat Germ Agglutinins

Topics

• Antigens, Tumor-Associated, Carbohydrate (analysis)
• Choroid Neoplasms (chemistry, pathology)
• Histocytochemistry
• Humans
• Lectins
• Male
• Melanoma (chemistry, pathology)
• Middle Aged
• Oligosaccharides (analysis)
• Plant Lectins
• Wheat Germ Agglutinins