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The assay of S-D-lactoylglutathione in biological systems.

Abstract
A procedure for the assay of S-D-lactoylglutathione, the physiological intermediate of the glyoxalase system, in biological systems is described, together with sample storage, sample processing, and statistical evaluation. Specimen data are presented. S-D-Lactoylglutathione was assayed by reverse-phase high-performance liquid chromatography (HPLC) with spectrophotometric detection of the thiolester chromophore at 233 nm. The biological sample was deproteinized with perchloric acid and partially purified by strong anion-exchange solid-phase extraction prior to HPLC. The limit of detection was 3.7 nmol, the recovery 49 +/- 4%, and the intra- and interbatch coefficients of variance 0.7 and 12%, respectively. The concentration of S-D-lactoylglutathione in whole blood from normal control human subjects was (mean +/- SD, nmol/ml whole blood) 16.5 +/- 4.4 (n = 8), and from diabetic patients 21.2 +/- 9.2 (n = 25), which is a significant increase (P < 0.05) from normal controls. The assay of S-D-lactoylglutathione is of increasing interest in studies of the elevation of glyoxalase metabolites in diabetes mellitus and in investigations of the antiproliferative activity of S-D-lactoylglutathione.
AuthorsA C McLellan, S A Phillips, P J Thornalley
JournalAnalytical biochemistry (Anal Biochem) Vol. 211 Issue 1 Pg. 37-43 (May 15 1993) ISSN: 0003-2697 [Print] United States
PMID8323036 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Glutathione
  • S-lactoylglutathione
Topics
  • Animals
  • Artifacts
  • Calibration
  • Chromatography, High Pressure Liquid (methods)
  • Diabetes Mellitus (metabolism)
  • Glutathione (analogs & derivatives, analysis)
  • Humans
  • Male
  • Rats
  • Rats, Wistar
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectrophotometry, Ultraviolet
  • Tumor Cells, Cultured

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