Sequence of a gene encoding a (poly ManA) alginate lyase active on Pseudomonas aeruginosa alginate.

Abstract

The recombinant plasmid pAL-A3 bears a (poly ManA) alginate lyase-encoding gene that originates from the marine bacterium ATCC 433367 (Brown et al., Appl. Environ. Microbiol. (1991) 57, 1870-1872). The alginate lyase produced by Escherichia coli TC4 harbouring pAL-A3 was purified to protein homogeneity and the corresponding gene sequenced, giving access to the first known primary structure of an alginate lyase. The 265-amino acid residue alginate lyase showed lytic activity on a Pseudomonas aeruginosa alginate isolated from a cystic fibrosis patient. Unexpectedly, the alginate lyase thus characterized differed from that isolated from the culture medium of the bacterium ATCC 433367 (Romeo and Preston, Biochemistry (1986) 25, 8385-8391).

• Authors: M Malissard, C Duez, M Guinand, M J Vacheron, G Michel, N Marty, B Joris, I Thamm, J M Ghuysen
• Journal: FEMS microbiology letters (FEMS Microbiol Lett) Vol. 110 Issue 1 Pg. 101-6 (Jun 01 1993) ISSN: 0378-1097 [Print] England
• PMID: 8319887 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Alginates
• Bacterial Proteins
• DNA, Bacterial
• Hexuronic Acids
• Polysaccharides, Bacterial
• Recombinant Fusion Proteins
• Glucuronic Acid
• Polysaccharide-Lyases
• poly(beta-D-mannuronate) lyase

Topics

• Alginates (metabolism)
• Amino Acid Sequence
• Bacterial Proteins (chemistry, genetics)
• Base Sequence
• Cloning, Molecular
• DNA, Bacterial (analysis)
• Electrophoresis, Polyacrylamide Gel
• Glucuronic Acid
• Hexuronic Acids
• Molecular Sequence Data
• Plasmids
• Polysaccharide-Lyases (chemistry, genetics)
• Polysaccharides, Bacterial (metabolism)
• Pseudomonas aeruginosa (chemistry)
• Recombinant Fusion Proteins
• Sequence Analysis, DNA