A
complement fixation (CF) test, a micro-immunofluorescence (micro-IF) test and an
enzyme immunoassay (EIA) using
Re-lipopolysaccharide as
antigen were compared in the diagnosis of chlamydial
infection in 136 mainly elderly patients hospitalized with community-acquired
pneumonia during a Chlamydia pneumoniae epidemic in Finland in 1986-1987.
Chlamydial pneumonia was diagnosed in 58 (42.6%) of the 136
pneumonia patients; 44 (75.9%) of them could be shown by micro-IF to be caused by Chlamydia pneumoniae, three by Chlamydia psittaci and four by Chlamydia spp. Only 5 (11.4%) of 44 patients with Chlamydia pneumoniae
pneumonia were
IgM-positive, indicating that the majority of cases were
reinfections. In this population of mainly elderly patients the CF test was insensitive, being positive in only 6 (10.3%) of 58 cases of
chlamydial pneumonia. The EIA detected 72.4% of cases and micro-IF 87.9% of cases (including
infections with Chlamydia pneumoniae, Chlamydia psittaci and Chlamydia spp.). In the EIA 77% of positive cases were positive in serum samples taken a week apart, whereas the corresponding figure for micro-IF was 50%. In micro-IF the measurement of
IgA antibody levels is recommended and
IgM-positive sera should be retested after removal of
IgG antibody to avoid false-positive findings due to presence of
rheumatoid factor. The collection of a third serum sample, for instance one month after onset, is also recommended, since half of the patients showed a diagnostic response in the micro-IF only in the sera taken one month apart.