Effects of pyrethroid insecticides on gap junctional intercellular communications in Balb/c3T3 cells by dye-transfer assay.

Abstract

The effects of fenvalerate, esfenvalerate, permethrin, cypermethrin, deltamethrin, p-chlorophenylisovaleric acid (CPIA, major metabolite of fenvalerate) and DDT, a liver tumor promoter, on gap junctional intercellular communication (GJIC) were examined in Balb/c3T3 cells by dye-transfer assay. Separate groups of Balb/c3T3 cells were exposed to the chemicals for 1 day. On the following day, GJIC was measured by counting the number of dye-transferring cells per injection of Lucifer Yellow under a fluorescent microscope. Fenvalerate, esfenvalerate, permethrin, cypermethrin, deltamethrin and DDT inhibited GJIC at noncytotoxic concentrations, while CPIA did not inhibit GJIC even at a cytotoxic concentration. It is concluded that the examined pyrethyroid insecticides, but not a metabolite, have inhibitory effects on GJIC in Balb/c3T3 cells.

• Authors: C Tateno, S Ito, M Tanaka, A Yoshitake
• Journal: Cell biology and toxicology (Cell Biol Toxicol) Vol. 9 Issue 3 Pg. 215-21 ( 1993) ISSN: 0742-2091 [Print] Switzerland
• PMID: 8299001 (Publication Type: Journal Article)

Chemical References

• Fluorescent Dyes
• Insecticides
• Isoquinolines
• Nitriles
• Pyrethrins
• cypermethrin
• decamethrin
• Permethrin
• lucifer yellow
• DDT
• fenvalerate

Topics

• 3T3 Cells (drug effects, metabolism)
• Animals
• Cell Communication (drug effects, physiology)
• DDT (toxicity)
• Fluorescent Dyes
• Gap Junctions (drug effects, metabolism)
• Insecticides (toxicity)
• Isoquinolines
• Mice
• Mice, Inbred BALB C
• Nitriles
• Permethrin
• Pyrethrins (metabolism, toxicity)