Minute virus of mice-induced modification of the murine DNA polymerase alpha-primase complex permits the salt-induced dissociation of 12S DNA primase and 10S DNA polymerase alpha components.

Abstract

DNA polymerase alpha-primase complexes in extracts of MVM-infected murine cells were dissociated in the presence of 0.3M KCl to generate a 12S DNA primase and a 10S DNA polymerase alpha that were readily separated by sedimentation in glycerol gradients. A 12S DNA polymerase alpha-primase complex refractory to dissociation in 0.3M KCl was identified in extracts of MVM-infected HeLa cells. In extracts of mock-infected murine and HeLa cells DNA primase and DNA polymerase alpha were not dissociated from each other in 0.3M KCl but remained in a stable complex that sedimented at 10S. We propose that a novel 12S DNA polymerase alpha-primase complex prone to disruption by salt is induced by MVM infection and that the DNA primase component of the complex is modified.

• Authors: J S Gupta, E A Faust
• Journal: Biochemistry and molecular biology international (Biochem Mol Biol Int) Vol. 31 Issue 4 Pg. 599-611 (Nov 1993) ISSN: 1039-9712 [Print] England
• PMID: 8298491 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Viral
• Salts
• DNA Primase
• RNA Nucleotidyltransferases
• DNA Polymerase II

Topics

• Animals
• Carcinoma, Ehrlich Tumor (enzymology)
• Cell Line, Transformed
• Cell Transformation, Viral (physiology)
• Cells, Cultured
• Cricetinae
• Cricetulus
• DNA Polymerase II (isolation & purification)
• DNA Primase
• DNA, Viral (biosynthesis)
• HeLa Cells
• Humans
• Mice
• Mice, Inbred BALB C
• Minute Virus of Mice (enzymology)
• RNA Nucleotidyltransferases (isolation & purification, metabolism)
• Salts