Antibiotic-induced release of endotoxin from bacteria in vitro.

The ability of cefotaxime, ciprofloxacin, piperacillin and tobramycin to cause release of endotoxin was examined in vitro with cultures of Enterobacter cloacae and Escherichia coli. Endotoxin was measured by a quantitative limulus amoebocyte lysate assay and its presence was confirmed by silver staining of the lipopolysaccharide moiety following SDS-PAGE. The morphology of the bacteria during antibiotic exposure was examined by scanning electronmicroscopy. Cefotaxime, ciprofloxacin and piperacillin caused significant endotoxin release, correlating with their ability to affect cell-wall morphology, causing filamentation, wall breakage and cell lysis. In contrast, little endotoxin was released when bacteria were exposed to tobramycin and no morphological changes were observed when bacteria were exposed to bactericidal concentrations of this aminoglycoside. Its antimicrobial spectrum and bactericidal activity make tobramycin an appropriate agent for treatment of sepsis caused by gram-negative bacteria and its lack of propensity to elicit excessive release of endotoxin may avoid exacerbation of endotoxin-related shock in sepsis.
AuthorsH A Crosby, J F Bion, C W Penn, T S Elliott
JournalJournal of medical microbiology (J Med Microbiol) Vol. 40 Issue 1 Pg. 23-30 (Jan 1994) ISSN: 0022-2615 [Print] SCOTLAND
PMID8289210 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Anti-Bacterial Agents
  • Endotoxins
  • Lipopolysaccharides
  • Ciprofloxacin
  • Cefotaxime
  • Tobramycin
  • Piperacillin
  • Anti-Bacterial Agents (pharmacology)
  • Cefotaxime (pharmacology)
  • Ciprofloxacin (pharmacology)
  • Colony Count, Microbial
  • Endotoxins (metabolism)
  • Enterobacter cloacae (drug effects, metabolism, ultrastructure)
  • Escherichia coli (drug effects, metabolism, ultrastructure)
  • Humans
  • Limulus Test
  • Lipopolysaccharides (analysis)
  • Microscopy, Electron, Scanning
  • Piperacillin (pharmacology)
  • Silver Staining
  • Tobramycin (pharmacology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: