Subcellular localization and antiviral activity of carminic acid/poly r(A-U) combinations.

Abstract

Carminic acid (CAR) enhances the antiviral activity of poly r(A-U) twelve-fold without increasing interferon induction, inactivating the vesicular stomatitis virus or inducing host cell cytotoxicity. Phase contrast photomicrographs of human foreskin fibroblasts (HSF) incubated with CAR alone, poly r(A-U) alone or with a CAR/poly r(A-U) combination illustrate that the CAR/poly r(A-U) combinations display altered subcellular distribution with the CAR being localized in the nucleoli and chromatin. Phase contrast and fluorescence photomicrographs of adriamycin (ADR)-treated and ADR/poly r(A-U)-treated HSF cells corroborate these findings. These results suggest that modulation of one or more nucleolar processes may be responsible for the enhanced antiviral activity.

• Authors: K Krabill, J M Jamison, J Gilloteaux, J L Summers
• Journal: Cell biology international (Cell Biol Int) Vol. 17 Issue 10 Pg. 919-34 (Oct 1993) ISSN: 1065-6995 [Print] England
• PMID: 8287022 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antiviral Agents
• Chromatin
• Drug Combinations
• Poly A-U
• Interferon-beta
• Doxorubicin
• Carmine

Topics

• Antiviral Agents (pharmacology)
• Carmine (analogs & derivatives, pharmacokinetics, pharmacology)
• Cell Nucleolus (metabolism)
• Cells, Cultured
• Chromatin (metabolism)
• Doxorubicin (pharmacology)
• Drug Combinations
• Drug Synergism
• Humans
• Interferon-beta (biosynthesis, physiology)
• Microscopy, Fluorescence
• Microscopy, Phase-Contrast
• Poly A-U (pharmacokinetics, pharmacology)
• Vesicular stomatitis Indiana virus (drug effects, growth & development)
• Viral Plaque Assay