Establishment and characterization of a feline large granular lymphoma cell line expressing interleukin 2 receptor alpha-chain.

Abstract

A feline large granular lymphocyte (LGL) cell line was established from a cat with an alimentary-form lymphoma. This cell line, designated as FGL, had many large azurophilic granules in the cytoplasm, which were typical to LGL cells. Proviral genome of feline leukemia virus was detected in the chromosomal DNA of FGL cells, and reverse transcriptase activity was also demonstrated in the culture supernatant. Furthermore, we found expression of interleukin 2 (IL-2) receptor alpha-chain on the cell surface of FGL and its natural killer activity against human erythroblastic leukemia cell line, K562.

• Authors: R Goitsuka, K Ohno, Y Matsumoto, N Hayashi, Y Momoi, Y Okamoto, T Watari, H Tsujimoto, A Hasegawa
• Journal: The Journal of veterinary medical science (J Vet Med Sci) Vol. 55 Issue 5 Pg. 863-5 (Oct 1993) ISSN: 0916-7250 [Print] Japan
• PMID: 8286547 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Neoplasm
• Macromolecular Substances
• Receptors, Interleukin-2

Topics

• Animals
• Blotting, Southern
• Cat Diseases
• Cats
• Cell Line
• Cell Survival
• Culture Techniques (methods)
• DNA, Neoplasm (analysis)
• Digestive System Neoplasms (metabolism, pathology, ultrastructure, veterinary)
• Humans
• Lymphoma (metabolism, pathology, ultrastructure, veterinary)
• Macromolecular Substances
• Microscopy, Electron
• Receptors, Interleukin-2 (analysis, biosynthesis)
• Tumor Cells, Cultured