Abstract |
The transforming growth factor-beta ( TGF-beta) superfamily plays a role in embryogenesis and regeneration. We have reported that osteogenic protein-1 (OP-1) promotes cell aggregation and induces the expression of the neural cell adhesion molecules N-CAM and L1 in proliferating neuroblastoma x glioma hybrid NG108-15 cells (Perides, G., Safran, R. M., Rueger, D. C., and Charness, M. E. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 10326-10330; Perides, G., Hu, G., Rueger, D. C., and Charness, M. E. (1993) J. Biol. Chem. 268, 25197-25205). Here we show that the structurally homologous bone morphogenetic proteins (BMP) BMP-2 and BMP-4 are 10-50-fold more potent in these actions than the subfamily comprising BMP-5, BMP-6, and OP-1 (BMP-7). In contrast, members of the TGF-beta subfamily, activin-A, inhibin-A, and 29 additional growth factors and cytokines did not induce N-CAM. The addition of serum to cells growing in serum-free medium caused a concentration-dependent increase in N-CAM and L1 expression; however, serum did not potentiate the induction of N-CAM and L1 by 40 ng/ml OP-1. These findings suggest the presence in NG108-15 cells of a BMP-2/BMP-4 receptor that discriminates subtle differences in structure among homologous members of the TGF-beta superfamily. An endogenous ligand for this receptor may be present in serum.
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Authors | G Perides, R M Safran, L A Downing, M E Charness |
Journal | The Journal of biological chemistry
(J Biol Chem)
Vol. 269
Issue 1
Pg. 765-70
(Jan 07 1994)
ISSN: 0021-9258 [Print] United States |
PMID | 8276880
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Bone Morphogenetic Proteins
- Cell Adhesion Molecules, Neuronal
- Growth Substances
- Leukocyte L1 Antigen Complex
- Proteins
- Transforming Growth Factor beta
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Topics |
- Animals
- Blood
- Bone Morphogenetic Proteins
- Cattle
- Cell Adhesion Molecules, Neuronal
(biosynthesis)
- Growth Substances
(physiology)
- Humans
- Leukocyte L1 Antigen Complex
- Multigene Family
- Proteins
(metabolism)
- Rats
- Transforming Growth Factor beta
(genetics, physiology)
- Tumor Cells, Cultured
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