Elevated content of phospholipase C-gamma 1 in colorectal cancer tissues.

Abstract

BACKGROUND:

Phospholipase C isozymes (PLC) play a role in ligand-mediated signal transduction for cellular activity, such as proliferation and differentiation. However, the biologic significance of their molecules in carcinogenesis or tumor progression is yet to be determined.

METHODS:

Using PLC isozyme-specific antibodies, the relative content of PLC in human colorectal carcinomas and in normal colonic mucosa was examined.

RESULTS:

Immunoreactive analysis revealed considerably higher levels of PLC-gamma 1 protein in 15 of 17 colorectal carcinomas and little difference in PLC-beta 1 or PLC-gamma 1 content compared with normal colorectal tissues. By radioimmunoassay and Western blotting, PLC-gamma 1 showed three-fold to fourfold more expression in carcinomas than that in normal tissues.

CONCLUSION:

Although factors that might influence the level of PLC-gamma 1 expression in colorectal carcinomas still remain obscure, the fact that most colorectal carcinomas display elevated levels of PLC-gamma 1 expression implies that PLC-gamma 1 may play an important role in proliferation of colorectal carcinoma cells.

• Authors: D Y Noh, Y H Lee, S S Kim, Y I Kim, S H Ryu, P G Suh, J G Park
• Journal: Cancer (Cancer) Vol. 73 Issue 1 Pg. 36-41 (Jan 01 1994) ISSN: 0008-543X [Print] United States
• PMID: 8275435 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Isoenzymes
• Type C Phospholipases
• Phospholipase C beta
• Phospholipase C delta
• Phospholipase C gamma

Topics

• Adult
• Aged
• Carcinoma (chemistry, genetics, pathology)
• Colon (chemistry, pathology)
• Colonic Neoplasms (chemistry, genetics, pathology)
• Female
• Gene Expression Regulation, Enzymologic
• Gene Expression Regulation, Neoplastic
• Humans
• Intestinal Mucosa (chemistry, pathology)
• Isoenzymes (analysis, chemistry, genetics)
• Male
• Middle Aged
• Phospholipase C beta
• Phospholipase C delta
• Phospholipase C gamma
• Rectal Neoplasms (chemistry, genetics, pathology)
• Signal Transduction (physiology)
• Type C Phospholipases (analysis, genetics)