Inflammatory mediator changes in cotton-top tamarins (CTT) after SC-41930 anti-colitic therapy.

Abstract

Use of the CTT model provides insight into the inflammatory mediator contribution in the pathogenesis of idiopathic colitis. To evaluate anti-colitic efficacy, the leukotriene B4 receptor antagonist and anti-inflammatory agent, SC-41930, was administered (10 mg/kg BW by gavage BID) for 8 weeks to CTTs with histologically confirmed persistent and defined active colitis. The inflammatory mediators LTB4, PGE2, TXB2, and PAF were assayed in colonic dialysate that was collected after 1 1/2 h from four CTTs pre-, mid-, and post-treatment, frozen at -70 degrees C, and analyzed by RIA after HPLC purification. LTB4 levels were lower at mid- and post-treatment and had little inter-animal variation post-treatment. PGE2 and PAF levels were elevated during SC-41930 treatment, but there was a trend towards lower thromboxane B2 levels. Reduced LTB4 (PMN degranulation and chemotaxis) and increased PGE2 (mucosal-protective effect) may, in part, explain the observed efficacy of SC-41930 in active tamarin colitis.

• Authors: N Clapp, M Henke, R Hansard, R Carson, R Walsh, D Widomski, C Anglin, D Fretland
• Journal: Agents and actions (Agents Actions) Vol. 39 Spec No Pg. C8-10 ( 1993) ISSN: 0065-4299 [Print] Switzerland
• PMID: 8273593 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Arachidonic Acids
• Benzopyrans
• Platelet Activating Factor
• SC 41930
• Leukotriene B4
• Thromboxane B2
• Dinoprostone

Topics

• Animals
• Anti-Inflammatory Agents, Non-Steroidal (pharmacology)
• Arachidonic Acids (metabolism)
• Benzopyrans (administration & dosage, pharmacology, therapeutic use)
• Colitis (drug therapy, metabolism)
• Colon (metabolism)
• Dinoprostone (metabolism)
• Disease Models, Animal
• Leukotriene B4 (antagonists & inhibitors, metabolism)
• Platelet Activating Factor (metabolism)
• Radioimmunoassay
• Saguinus
• Thromboxane B2 (metabolism)