[The isolation of monoclonal antibodies to hepatoma-associated nonhistone chromosomal proteins from rat kidneys].

Abstract

A monoclonal antibody (5D2) has been produced to a fraction of nonhistone proteins of kidney origin, associated with the rat hepatoma 27 and not obtained in normal liver. The 5D2 antibody was found to precipitate three proteins with molecular weights of 22-23 kDa. Indirect immunofluorescence studies on fixed hepatoma cell-preparations showed speckled nuclear staining patterns. In contrast, the 5D2 antibody was unreactive with intact hepatocytes.

• Authors: A B Grandilevskaia, D G Polyntsev, V P Kushner, V A Ivanov
• Journal: Tsitologiia (Tsitologiia) Vol. 35 Issue 9 Pg. 57-61 ( 1993) ISSN: 0041-3771 [Print] Russia (Federation)
• Vernacular Title: Poluchenie monoklonal'nykh antitel k negistonovym belkam khromatina pochek krys, assotsiirovannym s gepatomami.
• PMID: 8266582 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antibodies, Monoclonal
• Antigens, Heterophile
• Antigens, Neoplasm
• Chromosomal Proteins, Non-Histone

Topics

• Animals
• Antibodies, Monoclonal (analysis, isolation & purification)
• Antigens, Heterophile (immunology)
• Antigens, Neoplasm (immunology)
• Chromosomal Proteins, Non-Histone (immunology)
• Fluorescent Antibody Technique
• Hybridomas (immunology)
• Immunization
• Kidney (immunology)
• Liver (immunology)
• Liver Neoplasms, Experimental (immunology)
• Male
• Mice
• Mice, Inbred BALB C
• Molecular Weight
• Rats