Insulin-like growth factor-1 and its receptor mediate the autocrine proliferation of human ovarian carcinoma cell lines.

Abstract

BACKGROUND:

IGF-1 regulates the growth of diverse mammalian cell types including several human carcinoma cell lines. The IGF-1 receptor is a glycosylated heterodimer which, upon binding with IGF-1, undergoes tyrosine autophosphorylation. The autophosphorylation of the beta-receptor subunit is a strict requirement for its mitogenic properties.

EXPERIMENTAL DESIGN:

In this study, we have assessed the role of the IGF-1 receptor in the proliferation of ovarian carcinoma cell lines in culture. Effects of anti-sense and sense oligodeoxynucleotides to IGF-1 receptor RNA were tested.

RESULTS:

The human ovarian carcinoma cell lines OVCAR-3 and CaOV-3 both grew autonomously in serum-free medium, and their growth was further stimulated by the addition of IGF-1. Incubation of cells with anti-sense oligodeoxynucleotides corresponding to the IGF-1 receptor RNA markedly inhibited cell proliferation both in serum-free medium and in the presence of IGF-1. The inhibition of cell growth by the oligodeoxynucleotides corresponded to a reduction in the amount of detectable phosphorylated IGF-1 receptor.

CONCLUSIONS:

The findings indicate that IGF-1 and its specific receptor mediate the autocrine proliferation of human ovarian carcinoma cell lines.

• Authors: M Resnicoff, D Ambrose, D Coppola, R Rubin
• Journal: Laboratory investigation; a journal of technical methods and pathology (Lab Invest) Vol. 69 Issue 6 Pg. 756-60 (Dec 1993) ISSN: 0023-6837 [Print] United States
• PMID: 8264238 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Oligonucleotides, Antisense
• Insulin-Like Growth Factor I
• Receptor, IGF Type 1

Topics

• Animals
• Base Sequence
• Carcinoma (pathology)
• Cell Division
• Female
• Humans
• Insulin-Like Growth Factor I (physiology)
• Mice
• Molecular Sequence Data
• Oligonucleotides, Antisense (chemistry)
• Ovarian Neoplasms (pathology)
• Phosphorylation
• Receptor, IGF Type 1 (physiology)
• Signal Transduction
• Tumor Cells, Cultured