An in situ hybridization technique for the study of B19 human parvovirus replication in bone marrow cell cultures.

Abstract

An in situ hybridization technique using digoxigenin labelling was developed to study B19 infection. By using appropriate DNA probes, transcription of structural and non-structural genes was detected in bone marrow cell cultures. Such a simple system is useful to the study of B19-cell interactions in non-permissive cell lines.

• Authors: I Vassias, S Perol, L Coulombel, M C Thebault, P H Lagrange, F Morinet
• Journal: Journal of virological methods (J Virol Methods) Vol. 44 Issue 2-3 Pg. 329-38 (Oct 1993) ISSN: 0166-0934 [Print] Netherlands
• PMID: 8263124 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA Probes
• Digoxigenin

Topics

• Base Sequence
• Bone Marrow (microbiology)
• Cells, Cultured
• DNA Probes
• Digoxigenin
• Fluorescent Antibody Technique
• Genes, Viral
• Genome, Viral
• Humans
• In Situ Hybridization (methods)
• Leukocytes, Mononuclear
• Molecular Sequence Data
• Parvovirus B19, Human (genetics, physiology)
• Transcription, Genetic
• Virus Replication (genetics)