Modulation of mammalian cell proliferation by a modified tRNA base of bacterial origin.

Abstract

Addition of the q-base to q-deficient non-transformed mammalian cells stimulated their proliferation. The q-base also improved proliferation of some cancer-derived cell lines, but inhibited proliferation of others. The proliferation of HeLa-S3 carcinoma cells was stimulated by q under aerobic conditions, but was inhibited when the cells had shifted their energy metabolism towards glycolysis as the result of oxygen limitation. Q-deficient cells could not adapt their proliferation to the respective oxygen tension. The q-base stimulated the proliferation of non-transformed fibroblasts but inhibited proliferation of the same cell line, when aerobic glycolysis was increased after transformation with the ras gene. The results suggest that the q-base permits mammalian cells to adapt their proliferation to their specific metabolic state.

• Authors: W Langgut, T Reisser, S Nishimura, H Kersten
• Journal: FEBS letters (FEBS Lett) Vol. 336 Issue 1 Pg. 137-42 (Dec 20 1993) ISSN: 0014-5793 [Print] England
• PMID: 8262197 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Guanine
• queuine
• RNA, Transfer

Topics

• Animals
• Cell Division (drug effects)
• Cell Hypoxia
• Cell Line
• Cell Line, Transformed
• Cells, Cultured
• Energy Metabolism
• Glycolysis
• Guanine (analogs & derivatives, pharmacology)
• HeLa Cells
• Humans
• Mice
• Oncogenes
• RNA, Transfer (drug effects, physiology)
• Rats