| Abstract | Migration of very small amounts of immunoglobulin (20 ng) is restricted in agarose electrophoresis. Incorporation of a stable protein matrix (rabbit gamma globulin 1 mg/ml) in the agarose permits unrestricted migration so that immunoelectrophoresis of this quantity of radiolabelled antibody is possible. Very small amounts of radiolabelled and non-radiolabelled antibody were subjected to successful crossed immunoelectrophoresis through barriers of antigen under conditions which provide favorable ratios of antibody to antigen. These methods should be useful for studies of antibody eluted from tissue in acquired and autoimmune diseases associated with tissue bound immunoglobulin. |
| Authors | M L Weil, D L Tyrrell, E Norrby |
| Journal | Journal of immunological methods
(J Immunol Methods)
Vol. 24
Issue 1-2
Pg. 99-110
( 1978)
ISSN: 0022-1759 NETHERLANDS |
| PMID | 82591
(Publication Type: Journal Article)
|
| Chemical References |
- Antibodies, Viral
- Immunoglobulin G
- gamma-Globulins
|
| Topics |
- Animals
- Antibodies, Viral
- Electrophoresis, Agar Gel
- Humans
- Immunoelectrophoresis, Two-Dimensional
- Immunoglobulin G
- Measles
(immunology)
- Rabbits
- Subacute Sclerosing Panencephalitis
(immunology)
- gamma-Globulins
(metabolism)
|
