Calcium and skeletal muscle: a multiple regression on signal transduction mechanisms in burn trauma.

Abstract

The novelty of applying three-dimensional graphic capabilities involving area and vector changes was used to understand variations in inositol derivatives and their comodulating influence on calcium (Ca2+) in skeletal muscle under the duress of burn trauma. Burn injury was achieved by scalding of predefined areas (0, 20% and 50%) on the dorsal and ventral surfaces of mice. At day 21, 45Ca2+ influx and efflux procedures were performed. Through multiple regression, the dependency of intracellular Ca2+ was determined with respect to three polyinositol forms each representing independence simultaneously. The contribution of each of these parameters was assigned to a three-dimensional axis. Vector analysis determined the relationship of intracellular Ca2+ to each of the independent parameters in control, 20% and 50% burn groups. Such vector analysis allows for a clear visualization of the interrelationships that exist between secondary (viz, IP3) and tertiary (viz, Ca2+) messenger systems. This clear visualization may allow for a greater understanding of messenger systems that may lead to more effective treatment of the systemic effects of severe burn trauma.

• Authors: J F Tomera, K Lilford
• Journal: Methods and findings in experimental and clinical pharmacology (Methods Find Exp Clin Pharmacol) Vol. 15 Issue 7 Pg. 471-82 (Sep 1993) ISSN: 0379-0355 [Print] Spain
• PMID: 8255127 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Inositol Phosphates
• Calcium

Topics

• Animals
• Burns (metabolism, physiopathology)
• Calcium (metabolism)
• Inositol Phosphates (metabolism)
• Male
• Mice
• Mice, Inbred Strains
• Models, Biological
• Multivariate Analysis
• Muscles (metabolism, physiopathology)
• Regression Analysis
• Signal Transduction