c-N-ras is activated infrequently in canine malignant lymphoma.

Abstract

Activated c-N-ras alleles have been detected in human lymphoma specimens. The aim of the present study was to determine the frequency of c-N-ras mutational activation in canine malignant lymphoma. DNA was isolated from 28 canine malignant lymphoma specimens collected from 28 separate dogs and examined for c-N-ras mutations by polymerase chain reaction amplification and direct sequencing. The tumors were naturally occurring and derived from 20 dogs with known exposures to the phenoxy herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) and from 8 dogs with no known exposure to the herbicide. An oncogenically activating mutation was found in 1 dog without known 2,4-D exposure. The mutation was a 13th codon, second position transition that would result in a glycine-to-aspartate amino acid substitution. The results of this study demonstrate that, similar to the human, c-N-ras mutations are uncommon in dogs with malignant lymphoma and that there is no association between 2,4-D exposure and activation of c-N-ras in the dog.

• Authors: M D Edwards, K A Pazzi, P H Gumerlock, B R Madewell
• Journal: Toxicologic pathology (Toxicol Pathol) Vol. 21 Issue 3 Pg. 288-91 ( 1993) ISSN: 0192-6233 [Print] United States
• PMID: 8248717 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Carcinogens
• DNA, Neoplasm
• 2,4-Dichlorophenoxyacetic Acid

Topics

• 2,4-Dichlorophenoxyacetic Acid
• Animals
• Base Sequence
• Carcinogens
• DNA, Neoplasm (biosynthesis)
• Dog Diseases (genetics)
• Dogs
• Exons
• Genes, ras
• Histocytochemistry
• Lymphoma (chemically induced, genetics, veterinary)
• Mutation
• Polymerase Chain Reaction