Abstract |
Vitamin A ( retinol) is a prohormone that exerts its pleiotropic biological effects after conversion into multiple metabolites. In this report we describe the identification of three endogenous, retinolderived effector molecules, 14-hydroxy-retro-retinol (14-HRR), anhydroretinol (AR), and retinoic acid (RA) and a putative storage form of retinol, retinylesters (RE) in the human promyelocytic leukemia cell line HL-60. Exogenous application of the retinol metabolites in retinol-depleted serum-free cultures of HL-60 allowed the identification of unique cellular functions for each metabolite: 14-HRR is a growth factor for HL-60. AR is a functional antagonist of 14-HRR with growth-inhibiting activity, and RA is a potent inducer of granulocyte differentiation accompanied by growth arrest. Finally, intracellular RE serves as storage form allowing continuous production of 14-HRR when no external retinol is available.
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Authors | T M Eppinger, J Buck, U Hämmerling |
Journal | The Journal of experimental medicine
(J Exp Med)
Vol. 178
Issue 6
Pg. 1995-2005
(Dec 01 1993)
ISSN: 0022-1007 [Print] United States |
PMID | 8245778
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Diterpenes
- Growth Inhibitors
- Retinoids
- Vitamin A
- 14-hydroxy-4,14-retro-retinol
- anhydrovitamin A
- Tretinoin
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Topics |
- Cell Differentiation
(drug effects)
- Cell Division
(drug effects)
- Diterpenes
- Granulocytes
(cytology)
- Growth Inhibitors
- Humans
- In Vitro Techniques
- Retinoids
(metabolism)
- Tretinoin
(pharmacology)
- Tumor Cells, Cultured
- Vitamin A
(analogs & derivatives, pharmacology)
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