Efficient photodynamic action of Victoria blue BO against the human leukemic cell lines K-562 and TF-1.
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| Abstract |
Photodynamic induced cytotoxicity by Victoria blue BO (VB-BO), merocyanine 540 (MC540), Nile blue A (NB) and 4-tetrasulfonatophenyl-porphyrin (4-TSPP) has been studied on two human leukemic cell lines: K-562 and TF-1. Cells were incubated with dyes and irradiated with different doses of white light. Cell survival was assessed by propidium iodide (PI) staining using flow cytometry analysis. Concentrations of 5 x 10(-8) M VB-BO were found to kill 75% of cells, and a concentration of 1 x 10(-7) M induced more than 99% of cell killing. To obtain the same cytotoxic level, the presence of 2.6 x 10(-5) M of MC540 during irradiation was needed. Under the conditions used, NB was ineffective as a photosensitizer, although uptake studies showed that this dye was taken by the cells in much greater amounts than any other studied dye. Cell cycle distribution of TF-1 cells, surviving MC540 or VB-BO photosensitization has been studied by flow cytometry analysis after staining with Hoechst 33342 and PI. It was found that cells in G1 phase were slightly more resistant toward MC540- and VB-BO-mediated photosensitization than cells in other phases of the cell cycle.
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| Authors | M Fiedorowicz, J R Galindo, M Julliard, P Mannoni, M Chanon |
| Journal | Photochemistry and photobiology (Photochem Photobiol) Vol. 58 Issue 3 Pg. 356-61 (Sep 1993) ISSN: 0031-8655 [Print] United States |
| PMID | 8234469 (Publication Type: Journal Article) |
| Chemical References |
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