Cationic glycoproteins in sera of patients with acute infections identified as kappa light chain glycosylated IgG.

Abstract

In half of the sera from patients with acute bacterial infections and 15% of the sera from patients with acute viral infections glycoproteins were found that form a scalariform pattern in the cationic range upon isoelectric focusing. The cationic glycoproteins appeared with the clinical illness. After subsidence of the symptoms they disappeared within 4 to 6 weeks. The proteins were identified as immunoglobulin G (IgG) by determination of the NH2-terminal amino acid sequence. Remarkably, these IgG only contained light chains of the kappa type with high proportions of carbohydrates. Both, N-glycosidic- and O-glycosidic-bound glycans were present. The glycosylated light chains may render the cationic IgG multireactive. Thus, it may be part of an early nonspecific immune defense mechanism.

• Authors: K Ritter, A Wiebusch, M Mäder, H Eiffert, H Kratzin, R Thomssen
• Journal: Medical microbiology and immunology (Med Microbiol Immunol) Vol. 182 Issue 3 Pg. 107-17 (Jul 1993) ISSN: 0300-8584 [Print] Germany
• PMID: 8232066 (Publication Type: Journal Article)

Chemical References

• Cations
• Glycoproteins
• Immunoglobulin G
• Immunoglobulin Light Chains
• Immunoglobulin kappa-Chains

Topics

• Acute Disease
• Amino Acid Sequence
• Bacterial Infections (blood, immunology)
• Cations
• Glycoproteins (blood)
• Glycosylation
• Humans
• Immunoglobulin G (isolation & purification)
• Immunoglobulin Light Chains (isolation & purification)
• Immunoglobulin kappa-Chains (isolation & purification)
• Molecular Sequence Data
• Virus Diseases (blood, immunology)