Abstract |
We recently described construction and use of a beta-galactosidase expression cassette in isolating recombinant pseudorabies virus (PrV) mutants (Mettenleiter and Rauh, 1990). We report here the identification and exact quantitation of cells infected by these mutants using an assay based on the reaction of intracellular beta-galactosidase expressed during infection by the recombinant viruses with the fluorogenic substrate fluorescein di-beta-D-galactopyranoside (FDG) followed by detection of positive cells in flow cytometry (FACS-Gal assay; Nolan et al., 1988). The detection method is fast, sensitive, and reliable, and yields quantitative results on single cell basis.
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Authors | A Saalmüller, T C Mettenleiter |
Journal | Journal of virological methods
(J Virol Methods)
Vol. 44
Issue 1
Pg. 99-108
(Sep 1993)
ISSN: 0166-0934 [Print] Netherlands |
PMID | 8227283
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Recombinant Fusion Proteins
- beta-Galactosidase
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Topics |
- Animals
- Bone Marrow
(microbiology)
- Cattle
- Cells, Cultured
- Epithelium
- Flow Cytometry
- Fluorescent Antibody Technique
- Gene Expression Regulation
- Hematopoietic Stem Cells
(microbiology)
- Herpesvirus 1, Suid
(genetics, isolation & purification)
- Recombinant Fusion Proteins
(analysis)
- Swine
- beta-Galactosidase
(analysis)
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