Localization of the gene (RSN) coding for restin, a marker for Reed-Sternberg cells in Hodgkin's disease, to human chromosome band 12q24.3 and YAC cloning of the locus.

A novel 160-kDa intermediate filament associated protein, named restin (Reed-Sternberg intermediate filament associated protein), is specifically expressed in the malignant cells of Hodgkin's disease and anaplastic large cell lymphoma (Ki-1 lymphoma). The combination of chromosomal R-banding and fluorescence in situ hybridization (FISH) with the use of two fluorescent dyes, fluorescein isothiocyanate and propidium iodide, allowed simultaneous detection of the hybridized DNA sequence and chromosomal R-banding. By this technique, the gene coding for restin (RSN) was assigned to chromosome region 12q24.31-->q24.33, while localization of the alpha-2-macroglobulin receptor (A2MR) was refined to 12q13.1-->q13.3. To further analyze the restin gene, a 500-kb YAC clone containing the gene was isolated and analyzed. A restriction map of this area is presented.
AuthorsC Hilliker, J Delabie, F Speleman, G Bilbe, J Bruggen, F Van Leuven, H Van den Berghe
JournalCytogenetics and cell genetics (Cytogenet Cell Genet) Vol. 65 Issue 3 Pg. 172-6 ( 1994) ISSN: 0301-0171 [Print] SWITZERLAND
PMID8222754 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • DNA, Complementary
  • Intermediate Filament Proteins
  • Microtubule-Associated Proteins
  • Neoplasm Proteins
  • cytoplasmic linker protein 170
  • Chromosome Banding
  • Chromosome Mapping
  • Chromosomes, Artificial, Yeast
  • Chromosomes, Human, Pair 12
  • Cloning, Molecular
  • DNA, Complementary (genetics)
  • Humans
  • In Situ Hybridization, Fluorescence
  • Intermediate Filament Proteins (genetics)
  • Microtubule-Associated Proteins
  • Neoplasm Proteins (genetics)
  • Reed-Sternberg Cells (ultrastructure)
  • Restriction Mapping

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