Abstract |
A high-performance liquid chromatographic method is presented for the determination of urinary acylcarnitines. After solid phase extraction on silica columns the acylcarnitines are converted to 4'-bromophenacyl esters with 4'-bromophenacylbromide in the presence of N,N-diisopropylethylamine. Complete derivatization was achieved at 37 degrees C within 30 min. The 4'-bromophenacyl esters were separated by high-performance liquid chromatography on a Hypersil BDS C8 reversed-phase column with a binary gradient containing varying proportions of acetonitrile, water and 0.1 M triethylamine phosphate buffer. Essentially baseline separation was obtained with a standard mixture containing 4'-bromophenacyl esters of carnitine and synthetic acylcarnitines of increasing chain length ranging from acetyl- to palmitoylcarnitine. The method was used to obtain urinary acylcarnitine profiles from patients with propionic, methylmalonic and isovaleric acidemia and with medium-chain and multiple acyl-CoA dehydrogenase deficiency. Quantification of the acylcarnitines was achieved using undecanoylcarnitine as internal standard.
|
Authors | B J Poorthuis, T Jille-Vlcková, W Onkenhout |
Journal | Clinica chimica acta; international journal of clinical chemistry
(Clin Chim Acta)
Vol. 216
Issue 1-2
Pg. 53-61
(Jul 16 1993)
ISSN: 0009-8981 [Print] Netherlands |
PMID | 8222273
(Publication Type: Journal Article)
|
Chemical References |
- Acetophenones
- Ethylamines
- Fatty Acids
- undecanoylcarnitine
- N,N-diisopropylethylamine
- Acetylcarnitine
- Acyl-CoA Dehydrogenases
- Phospholipases A
- 4-bromophenacyl bromide
- Carnitine
|
Topics |
- Acetophenones
(pharmacology)
- Acetylcarnitine
(analogs & derivatives, urine)
- Acyl-CoA Dehydrogenases
(deficiency)
- Amino Acid Metabolism, Inborn Errors
(urine)
- Carnitine
(metabolism, urine)
- Child
- Child, Preschool
- Chromatography, High Pressure Liquid
- Ethylamines
- Fatty Acids
(metabolism)
- Female
- Humans
- Infant
- Lipid Metabolism, Inborn Errors
(urine)
- Male
- Phospholipases A
(antagonists & inhibitors)
|