Tissue-specific and wound-inducible pattern of expression of the mannopine synthase promoter is determined by the interaction between positive and negative cis-regulatory elements.
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| Abstract |
In transgenic tobacco plants the regulatory region of the Agrobacterium genes involved in mannopine synthesis (pmas) directs expression in vascular tissues and is induced upon wounding. To identify cis-acting sequences required for the cell-type-specific and wound-inducible expression of this regulatory region, the expression pattern of chimeric genes in which 5' upstream deletions of the pmas 1' promoter direct the expression of the beta-glucuronidase coding sequence, was analyzed in transgenic tobacco plants. It was found that the pmas1' promoter is specifically expressed in phloem cells and that this tissue-specific pattern of expression is the result of the interaction of at least two negative regulatory elements with a positive element that directs expression in many cell types. It was also found that wound induction is mediated by two different mechanisms, one of them involving a dramatic change in tissue-specific expression.
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| Authors | A Guevara-García, G Mosqueda-Cano, G Argüello-Astorga, J Simpson, L Herrera-Estrella |
| Journal | The Plant journal : for cell and molecular biology (Plant J) Vol. 4 Issue 3 Pg. 495-505 (Sep 1993) ISSN: 0960-7412 [Print] England |
| PMID | 8220492 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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