In vitro and in vivo mutagenicity studies on taurohyodeoxycholic acid.

Abstract

The mutagenicity of a new biliary acid, taurohyodeoxycholic acid (THDCA, Io, Praxis, CAS 2958-04-5), was assayed by using 5 different tests. The Ames test (reverse mutation assay on Salmonella typhimurium) and the DNA damage and repair test (in Saccharomyces cerevisiae) allowed to study the genetic THDCA-induced mutations in prokaryotes and eukaryotes (doses of 100, 200 and 400 micrograms/plate or 100, 200 and 400 micrograms/ml, respectively). In vivo and in vitro chromosomal aberrations were studied by using micronucleus test in mice (doses of 100, 220 and 500 mg/kg in oral study and 50, 100 and 200 mg/kg in subcutaneous study) and human lymphocytes cytogenetic test (doses of 50, 100, 220 and 500 micrograms/ml of THDCA). At last the host-mediated assay was performed on THDCA-treated mice (following oral or subcutaneous administration) in order to test the potential mutagenic activity of its metabolites on a S. typhimurium strain. The results obtained in these studies showed that THDCA did not induce any signs of promutagenic, mutagenic or clastogenic direct or metabolite-mediated activity.

• Authors: A S Tripodi, F Feletti, R Molteni, R Germogli
• Journal: Arzneimittel-Forschung (Arzneimittelforschung) Vol. 43 Issue 8 Pg. 897-903 (Aug 1993) ISSN: 0004-4172 [Print] Germany
• PMID: 8216450 (Publication Type: Journal Article)

Chemical References

• Cholagogues and Choleretics
• Culture Media
• Mutagens
• taurohyodeoxycholic acid
• Taurodeoxycholic Acid

Topics

• Adult
• Animals
• Cholagogues and Choleretics (toxicity)
• Chromosome Aberrations
• Crossing Over, Genetic (drug effects)
• Culture Media
• DNA Damage
• DNA Repair
• Female
• Gene Conversion (drug effects)
• Humans
• In Vitro Techniques
• Lymphocytes (ultrastructure)
• Male
• Mice
• Micronucleus Tests
• Middle Aged
• Mitosis (drug effects)
• Mutagenicity Tests
• Mutagens (toxicity)
• Rats
• Saccharomyces cerevisiae (drug effects, genetics)
• Salmonella typhimurium (drug effects, genetics)
• Taurodeoxycholic Acid (analogs & derivatives, toxicity)