Quantification of free carnitine, individual short- and medium-chain acylcarnitines, and total carnitine in plasma by high-performance liquid chromatography.

Abstract

This paper describes a method for the quantitative determination of free carnitine, acetylcarnitine, propionylcarnitine, hexanoylcarnitine, octanoylcarnitine, and total carnitine in plasma. Carnitine and acylcarnitines were extracted from 100 microliters of plasma with acetonitrile/methanol and isolated using 0.5-ml columns of silica gel. Samples were then derivatized with 4'-bromophenacyl trifluoromethanesulfonate and quantified by high-performance liquid chromatography with detection at 260 nm. Carnitine and acylcarnitines were quantified in normal human plasma and the plasma of patients diagnosed with methylmalonic aciduria, propionic acidemia, and medium-chain acyl-CoA dehydrogenase deficiency.

• Authors: P E Minkler, C L Hoppel
• Journal: Analytical biochemistry (Anal Biochem) Vol. 212 Issue 2 Pg. 510-8 (Aug 01 1993) ISSN: 0003-2697 [Print] United States
• PMID: 8214594 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Propionates
• hexanoylcarnitine
• propionylcarnitine
• Acetylcarnitine
• Methylmalonic Acid
• Acyl-CoA Dehydrogenases
• Acyl-CoA Dehydrogenase
• propionic acid
• octanoylcarnitine
• Carnitine

Topics

• Acetylcarnitine (blood)
• Acyl-CoA Dehydrogenase
• Acyl-CoA Dehydrogenases (deficiency)
• Acylation
• Carnitine (analogs & derivatives, blood)
• Chromatography, High Pressure Liquid
• Humans
• Methylmalonic Acid (urine)
• Molecular Weight
• Propionates (blood)
• Reference Standards
• Reproducibility of Results