Oral vaccination of racoons (Procyon lotor) with baculovirus-expressed rabies virus glycoprotein.

Abstract

Successful field oral vaccination and protection against viral diseases have so far been achieved only with live-attenuated or live-recombinant virus vaccines. In this communication, we present data that demonstrate that a glycoprotein derived from recombinant baculovirus-infected insect cells is efficacious as an oral vaccine. The glycoprotein (G) of rabies virus (Evelyn Rokitnicki Abelseth strain) was abundantly expressed in a baculovirus expression system and oral vaccination of racoons with the baculovirus-expressed G protein resulted in the production of rabies virus-neutralizing antibodies and protection against a lethal challenge with a street rabies virus. The potential for using the baculovirus-expressed G protein for oral immunization of wildlife is discussed.

• Authors: Z F Fu, C E Rupprecht, B Dietzschold, P Saikumar, H S Niu, I Babka, W H Wunner, H Koprowski
• Journal: Vaccine (Vaccine) Vol. 11 Issue 9 Pg. 925-8 ( 1993) ISSN: 0264-410X [Print] Netherlands
• PMID: 8212838 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Viral
• Antigens, Viral
• Glycoproteins
• Rabies Vaccines
• Recombinant Fusion Proteins
• Vaccines, Synthetic
• Viral Envelope Proteins
• glycoprotein G, Rabies virus

Topics

• Administration, Oral
• Animals
• Antibodies, Viral (biosynthesis, immunology)
• Antigens, Viral
• Baculoviridae
• Cells, Cultured
• Cricetinae
• Glycoproteins (administration & dosage, immunology)
• Kidney
• Mesocricetus
• Moths
• Rabies (prevention & control)
• Rabies Vaccines (administration & dosage, immunology)
• Rabies virus (immunology)
• Raccoons (immunology)
• Recombinant Fusion Proteins (immunology)
• Vaccination (methods, veterinary)
• Vaccines, Synthetic (administration & dosage, immunology)
• Viral Envelope Proteins (administration & dosage, immunology)