Induction of T-cell immunity to oligosaccharide antigens immobilized on crystalline bacterial surface layers (S-layers).

Abstract

Immunization of Balb/c mice with conjugates of oligosaccharide haptens and crystalline bacterial surface-layer proteins (S-layers) primed the mice for a strong, hapten-specific, delayed-type hypersensitivity (DTH) response. Conjugates of haptens with bovine serum albumin produced only weak DTH responses but, when mixed with aluminium hydroxide, elicited DTH responses comparable to those against S-layer conjugates. Surface-layer conjugates also elicited strong anti-hapten DTH responses when administered by an oral/nasal route. Apparently, the natural assembly of S-layer proteins into large, two-dimensional arrays endows them with intrinsic adjuvant properties.

• Authors: R H Smith, P Messner, L R Lamontagne, U B Sleytr, F M Unger
• Journal: Vaccine (Vaccine) Vol. 11 Issue 9 Pg. 919-24 ( 1993) ISSN: 0264-410X [Print] Netherlands
• PMID: 8212837 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adjuvants, Immunologic
• Antigens
• Bacterial Proteins
• Drug Carriers
• Haptens
• Oligosaccharides
• Serum Albumin, Bovine
• Aluminum Hydroxide
• Cyclophosphamide

Topics

• Adjuvants, Immunologic
• Administration, Oral
• Aluminum Hydroxide (immunology)
• Animals
• Antigens (administration & dosage, immunology)
• Bacterial Proteins (immunology, isolation & purification)
• Carbohydrate Sequence
• Cell Wall (chemistry, immunology)
• Crystallization
• Cyclophosphamide (pharmacology)
• Drug Carriers
• Female
• Haptens (administration & dosage, immunology)
• Hypersensitivity, Delayed (immunology)
• Immunity, Cellular (drug effects)
• Immunization (methods)
• Immunotherapy, Adoptive
• Lymphocyte Activation (drug effects)
• Mice
• Mice, Inbred BALB C (immunology)
• Molecular Sequence Data
• Oligosaccharides (administration & dosage, immunology)
• Serum Albumin, Bovine (immunology)
• T-Lymphocytes (immunology)