Abstract |
Immunological activity of synthetic cell wall peptidoglycan subunits was examined in guinea pigs and mice. It was concluded that the minimal adjuvant-active subunit of cell wall peptidoglycan for the induction of delayed-type hypersensitivity to monoazobenzenearsonate-N- acetyl-L-tyrosine and for circulating-antibody formation to bacterial alpha-amylase and the thymus-independent antigen DNP-Ficoll was N-acetylmuramyldipeptide, MurNAc-L-Ala-D-isoGln. N-acetylmuramyldipeptide and 6-O-stearoyl-N-acetylmuramyldipeptide showed no adjuvant activity in the generation of cell-mediated cytotoxic effector cells in the spleens of C57Bl/6J mice after in vivo immunization with the allogeneic antigen mastocytoma P815-X2 cells, but N-acetylmuramyldipeptide showed adjuvant activity after in vitro sensitization of C57Bl/6J mouse spleen cells to the alloantigen mitomycin C-treated DBA/2 mouse spleen cells. It was also shown that 6-O-stearoylation of N-acetylmuramylpeptide could not potentiate the adjuvant activity of N-acetylmuramyldipeptide. Mitogenic and antitumor activities were not observed in either N-acetylmuramyldipeptide or 6-O-stearoyl-N-acetylmuramyldipeptide in mouse systems.
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Authors | I Azuma, K Sugimura, T Taniyama, M Yamawaki, Y Yamamura |
Journal | Infection and immunity
(Infect Immun)
Vol. 14
Issue 1
Pg. 18-27
(Jul 1976)
ISSN: 0019-9567 [Print] United States |
PMID | 820642
(Publication Type: Journal Article)
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Chemical References |
- Adjuvants, Immunologic
- Dipeptides
- Mitogens
- Peptides
- Peptidoglycan
- Ficoll
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Topics |
- Adjuvants, Immunologic
- Animals
- Antibody Formation
- Cell Wall
(immunology)
- Chemical Phenomena
- Chemistry
- Culture Techniques
- Cytotoxicity Tests, Immunologic
- Dipeptides
(immunology, pharmacology)
- Ficoll
(immunology)
- Guinea Pigs
- Hypersensitivity, Delayed
(chemically induced)
- Mast-Cell Sarcoma
- Mice
- Mitogens
- Mycobacterium bovis
(immunology)
- Peptides
(immunology, pharmacology)
- Peptidoglycan
(immunology)
- Spleen
(immunology)
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