1. We investigated the mechanism of
hypoxia-induced depression of
gamma-aminobutyric acid-A (GABAA)-mediated inhibitory postsynaptic currents (IPSCs) in CA1 neurons of hippocampal slices from 21- to 28-day-old rats. Cells were examined by whole-cell patch-clamp recording and
hypoxia was induced by switching perfusion of the slice from oxygenated artificial cerebral spinal fluid (ACSF) to ACSF saturated with 95% N2-5% CO2. 2. Synaptic responses evoked by stimulation of the Schaffer collateral-commissural projection at a fixed holding potential (VH = -60 mV) during
anoxia revealed that the IPSC appeared more sensitive than the excitatory postsynaptic current to
anoxia-induced depression. All subsequent studies examined the GABAA-mediated IPSC synaptic responses in isolation by direct stimulation of
GABA interneurons in the stratum radiatum in the presence of extracellular 3-(2-carboxypiperazine-4-yl)propyl-1-phosphonic
acid (
CPP) (20 microM) and
6-cyano-7-nitroquinoxaline-2,3-dione (
CNQX) (50 microM) to block glutamatergic currents and intracellular
QX-314 (
lidocaine N-ethyl
bromide, 1 mM) to block GABAB-mediated currents. When studied in this manner (VH = -60 mV) the GABAA-mediated IPSC appeared to change from an outward to inward current after exposure to
anoxia. 3. The current-voltage relationship of GABAA-mediated IPSCs revealed that these changes resulted from a positive shift in the IPSC reversal potential without a significant change in the conductance. Thus under patch clamp apparent IPSC inhibition may result from a decrease in the extracellular concentration of
chloride ions. Similar findings were observed with micropipettes that contained high intracellular
chloride concentrations. 4. Miniature spontaneous IPSCs were examined in the presence of
tetrodotoxin (1 microM) with micropipettes containing high intracellular
chloride concentrations. The miniature IPSCs (mIPSCs) appeared as spontaneous transient inward currents. Consistent with an
anoxia-induced decrease in extracellular
chloride, the mean amplitude of the mIPSCs increased after the onset of
anoxia. A significant decrease in rise and decay time was also noted during
anoxia. The frequency of the mIPSCs also increased by approximately 300%. 5. The resting input resistance of the cells was examined by measuring the current resulting from a 20-mV hyperpolarizing pulse. A significant reduction in resistance was observed 2 min after the onset of
anoxia. This still occurred, although to a lesser degree, in the presence of glutamatergic blockers (20 microM
CPP plus 50 microM
CNQX). In the presence of both GABAergic (
picrotoxin, 100 microM) and glutamatergic blockers no significant reduction in resting input resistance was apparent after 2 min of
anoxia.(ABSTRACT TRUNCATED AT 400 WORDS)