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Platelet-mediated transformation of mtDNA-less human cells: analysis of phenotypic variability among clones from normal individuals--and complementation behavior of the tRNALys mutation causing myoclonic epilepsy and ragged red fibers.

Abstract
In the present work, we demonstrate the possibility of using human blood platelets as mitochondrial donors for the repopulation of mtDNA-less (rho 0) cells. The noninvasive nature of platelet isolation, combined with the prolonged viability of platelet mitochondria and the simplicity and efficiency of the mitochondria-transfer procedure, has substantially increased the applicability of the rho 0 cell transformation approach for mitochondrial genetic analysis and for the study of mtDNA-linked diseases. This approach has been applied to platelets from several normal human individuals and one individual affected by the myoclonic-epilepsy-and-ragged-red-fibers (MERRF) encephalomyopathy. A certain variability in respiratory capacity was observed among the platelet-derived rho 0 cell transformants from a given normal subject, and it was shown to be unrelated to their mtDNA content. The results of sequential transfer of mitochondria from selected transformants into a rho 0 cell line different from the first rho 0 acceptor strongly suggest that this variability reflected, at least in part, differences in nuclear gene content and/or activity among the original recipient cells. A much greater variability in respiratory capacity was observed among the transformants derived from the MERRF patient and was found to be related to the presence and amount of the mitochondrial tRNALys mutation associated with the MERRF syndrome. An analysis of the relationship between proportion of mtDNA carrying the MERRF mutation and degree of respiratory activity in various transformants derived from the MERRF patient revealed an unusual complementation behavior of the tRNALys mutation, possibly reflecting the distribution of mutant mtDNA among the platelet mitochondria.
AuthorsA Chomyn, S T Lai, R Shakeley, N Bresolin, G Scarlato, G Attardi
JournalAmerican journal of human genetics (Am J Hum Genet) Vol. 54 Issue 6 Pg. 966-74 (Jun 1994) ISSN: 0002-9297 [Print] United States
PMID8198140 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • DNA, Mitochondrial
  • RNA, Transfer, Lys
  • Electron Transport Complex IV
Topics
  • Adult
  • Aged
  • Blood Platelets
  • Cell Fusion
  • Cell Line
  • DNA Mutational Analysis
  • DNA, Mitochondrial (analysis, genetics)
  • Electron Transport Complex IV (analysis)
  • Female
  • Genetic Complementation Test
  • Humans
  • MERRF Syndrome (genetics)
  • Male
  • Mitochondria (metabolism)
  • Mutation (genetics)
  • Oxygen Consumption
  • Phenotype
  • RNA, Transfer, Lys (genetics)
  • Transformation, Genetic

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