Difference in sero-diagnostic values among KL-6-associated mucins classified as cluster 9.

Abstract

KL-6 classified as Cluster 9 (MUC-I) is a circulating high-molecular-weight mucin-like molecule. Serum level of KL-6 was measured by a sandwich assay using KL-6 antibody as not only a catcher but also as a tracer. We established 2 additional monoclonal antibodies (MAbs), LISA 101 and EH-123, reacting with KL-6 epitopes different from the epitope recognized by KL-6 antibody. The KL-6-associated mucins detected by the sandwich assay using LISA 101 or EH-123 antibody as a catcher and KL-6 antibody as a tracer were designated as LISA 1-6 and CAM 123-6 respectively. The diagnostic values as the serum markers of KL-6, LISA 1-6 and CAM 123-6 were evaluated measuring their levels in the same serum from healthy individuals and from patients with pulmonary, pancreatic and breast adenocarcinomas. KL-6 was increased abnormally at high rates of more than 50% in pancreatic cancer and in benign lung diseases, LISA 1-6 only in pancreatic cancer, and CAM 123-6 only in pulmonary adenocarcinoma. In benign lung diseases, however, LISA 1-6 and CAM 123-6 were increased abnormally at the rates of only 5.3% and 0% respectively. These observations clearly indicate that LISA 1-6 and CAM 123-6 constitute a part of KL-6, but that they are superior to KL-6 as tumor markers for pancreatic cancer and for pulmonary adenocarcinoma respectively, because of their much lower false-positive rates.

• Authors: N Kohno, Y Inoue, H Hamada, S Fujioka, S Fujino, A Yokoyama, K Hiwada, N Ueda, M Akiyama
• Journal: International journal of cancer. Supplement = Journal international du cancer. Supplement (Int J Cancer Suppl) Vol. 8 Pg. 81-3 ( 1994) ISSN: 0898-6924 [Print] United States
• PMID: 8194900 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens
• Antigens, Neoplasm
• Biomarkers, Tumor
• Glycoproteins
• MUC1 protein, human
• Mucin-1
• Mucins

Topics

• Adenocarcinoma (blood, diagnosis)
• Antigens (blood)
• Antigens, Neoplasm
• Biomarkers, Tumor (blood)
• Breast Neoplasms (blood, diagnosis)
• Diagnosis, Differential
• Enzyme-Linked Immunosorbent Assay
• Female
• Glycoproteins (blood)
• Humans
• Lung Diseases (blood)
• Lung Neoplasms (blood, diagnosis)
• Mucin-1
• Mucins (blood)
• Pancreatic Neoplasms (blood, diagnosis)
• Reference Values