Abstract |
The chemokines are a large group of cytokines that are recognized to be important mediators of inflammation. In this study we show that the human mast cell leukemia line HMC-1 is a source of multiple chemokines, including I-309, monocyte chemoattractant protein 1, macrophage inflammatory protein-1 alpha, macrophage inflammatory protein-1 beta, RANTES, and interleukin-8. I-309 and MCP-1 transcripts are expressed at low levels in unstimulated HMC-1. However, phorbol ester treatment up-regulates these and other chemokine transcript levels and also up-regulates chemokine protein synthesis and secretion. Induction of chemokine transcripts in HMC-1 requires de novo protein synthesis. We compared the effects of anti-inflammatory glucocorticoids on the expression of chemokine genes in HMC-1 to their effects in activated T-cells. We find that methyl- prednisolone reduces MCP-1 but not other chemokine transcripts in HMC-1, even though there are distinct and more general effects on chemokine transcripts in activated T-cells. These effects are attributed to inhibition of transcription rather than transcript stability. Our results suggest that human mast cells may be a source of multiple chemokines, that glucocorticoids may inhibit the expression of only a subset of these chemokines, and that mast cells and T-cell chemokine expression may occur via distinct regulatory pathways.
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Authors | R S Selvan, J H Butterfield, M S Krangel |
Journal | The Journal of biological chemistry
(J Biol Chem)
Vol. 269
Issue 19
Pg. 13893-8
(May 13 1994)
ISSN: 0021-9258 [Print] United States |
PMID | 8188667
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cytokines
- Glucocorticoids
- RNA, Messenger
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Topics |
- Cytokines
(genetics)
- Gene Expression Regulation, Neoplastic
- Glucocorticoids
(pharmacology)
- Humans
- Leukemia, Mast-Cell
(genetics)
- Lymphocyte Activation
- Mast Cells
(metabolism)
- RNA, Messenger
(metabolism)
- T-Lymphocytes
(drug effects, immunology, metabolism)
- Tumor Cells, Cultured
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