Abstract |
Carnitine dehydratase from Escherichia coli O44 K74 is an inducible enzyme detectable in cells grown anaerobically in the presence of L-(-)-carnitine or crotonobetaine. The purified enzyme catalyzes the dehydration of L-(-)-carnitine to crotonobetaine (H. Jung, K. Jung, and H.-P. Kleber, Biochim. Biophys. Acta 1003:270-276, 1989). The caiB gene, encoding carnitine dehydratase, was isolated by oligonucleotide screening from a genomic library of E. coli O44 K74. The caiB gene is 1,215 bp long, and it encodes a protein of 405 amino acids with a predicted M(r) of 45,074. The identity of the gene product was first assessed by its comigration in sodium dodecyl sulfate- polyacrylamide gels with the purified enzyme after overexpression in the pT7 system and by its enzymatic activity. Moreover, the N-terminal amino acid sequence of the purified protein was found to be identical to that predicted from the gene sequence. Northern ( RNA) analysis showed that caiB is likely to be cotranscribed with at least one other gene. This other gene could be the gene encoding a 47-kDa protein, which was overexpressed upstream of caiB.
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Authors | K Eichler, W H Schunck, H P Kleber, M A Mandrand-Berthelot |
Journal | Journal of bacteriology
(J Bacteriol)
Vol. 176
Issue 10
Pg. 2970-5
(May 1994)
ISSN: 0021-9193 [Print] United States |
PMID | 8188598
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- RNA, Messenger
- Recombinant Proteins
- Acyltransferases
- crotonobetainyl-CoA carnitine coenzyme A transferase
- Hydro-Lyases
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Topics |
- Acyltransferases
- Amino Acid Sequence
- Base Sequence
- Blotting, Northern
- Cloning, Molecular
- Escherichia coli
(genetics)
- Genes, Bacterial
(genetics)
- Hydro-Lyases
(analysis, biosynthesis, genetics)
- Molecular Sequence Data
- RNA, Messenger
(genetics)
- Recombinant Proteins
(biosynthesis)
- Restriction Mapping
- Sequence Analysis
- Transcription, Genetic
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