Monoclonal antibody Ber-EP4: its use in the differential diagnosis of malignant mesothelioma and carcinoma in cell blocks of malignant effusions and FNA specimens.

Abstract

Formol sublimate-fixed cell blocks derived from 129 malignant pleural (and some peritoneal) effusions, 8 benign effusions with reactive mesothelial cells, and 23 FNA specimens, were immunostained with monoclonal antibody Ber-EP4 to assess its ability to distinguish malignant mesothelioma (MM) from carcinoma. Only 2 of 44 (4%) well-characterized MM were Ber-EP4+, while none of 8 benign mesothelial proliferations reacted with the antibody. Fifty-seven percent of 23 pulmonary adenocarcinomas (AC) and 60% of 43 pulmonary carcinomas of all other histological types were Ber-EP4+. Of 40 metastatic AC originating from breast, gastrointestinal tract, ovary, endometrium, and kidney, 80% were Ber-EP4+. The predictive value of positive Ber-EP4 staining in distinguishing AC from MM was 96%. The predictive value of a negative Ber-EP4 in excluding MM was 70%, when the differential diagnosis was adenocarcinoma. These results suggest that Ber-EP4 is helpful in differentiating MM and AC if used together with other discriminating antibodies.

• Authors: B Maguire, D Whitaker, S Carrello, D Spagnolo
• Journal: Diagnostic cytopathology (Diagn Cytopathol) Vol. 10 Issue 2 Pg. 130-4 ( 1994) ISSN: 8755-1039 [Print] United States
• PMID: 8187591 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal

Topics

• Antibodies, Monoclonal
• Ascitic Fluid (pathology)
• Biopsy, Needle
• Carcinoma (pathology, secondary)
• Humans
• Lung Neoplasms (pathology)
• Mesothelioma (pathology)
• Peritoneal Neoplasms (pathology, secondary)
• Pleural Effusion, Malignant (pathology)
• Pleural Neoplasms (pathology, secondary)