Mechanism of hemolysis of canine erythrocytes induced by L-sorbose.

Abstract

The cause of species difference in the susceptibility of erythrocytes to L-sorbose, and the difference in the hemolytic effect of sorbose on high potassium-containing (HK) and low potassium-containing (LK) canine erythrocytes were examined. L-Sorbose was phosphorylated in canine erythrocytes, but not in human erythrocytes. Furthermore, sorbose-1-phosphate, a metabolite of L-sorbose, strongly inhibited the hexokinase of LK canine erythrocytes, but not that of HK canine erythrocytes. These results strongly indicated that inhibition of hexokinase by sorbose-1-phosphate in LK erythrocytes induced severe glycolytic limitation in these cells, resulting in hemolysis, and that HK erythrocytes are resistant to sorbose-induced hemolysis because these cells have a high hexokinase activity.

• Authors: I Goto, M Inaba, T Shimizu, Y Maede
• Journal: American journal of veterinary research (Am J Vet Res) Vol. 55 Issue 2 Pg. 291-4 (Feb 1994) ISSN: 0002-9645 [Print] United States
• PMID: 8172422 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Hexosephosphates
• sorbose-1-phosphate
• Hexokinase
• Sorbose
• Potassium

Topics

• Animals
• Dogs
• Erythrocytes (drug effects, physiology)
• Hemolysis (drug effects)
• Hexokinase (antagonists & inhibitors, blood)
• Hexosephosphates (pharmacology)
• In Vitro Techniques
• Kinetics
• Phosphorylation
• Potassium (blood)
• Sorbose (analogs & derivatives, blood, pharmacology)